Polymorphism of HIF-1A (rs11549465) and VEGFA (rs2010963) genes and the immune status in the dust lung pathology miners working at the coal enterprises in the South of Kuzbass

Abstract

The aim of the study was to study the polymorphism of HIF-1A (rs11549465) and VEGFA (rs2010963) genes and their association with immunological parameters among the miners with lung dust pathology in the Kemerovo region. Material and methods. 200 Kuzbass miners aged from 39 to 58 years, working in the primary occupations with a high risk of occupational pathology, were examined. All the subjects were divided into two groups: the 1st group included 130 cases with a proven diagnosis of dust lung pathology. The 2nd group was a control one represented by 70 workers in the same sanitary and hygienic conditions but without occupational diseases. Polymorphisms of the HIF-1A (rs11549465) and VEGFA (rs2010963) genes were studied by the method of real-time polymerase chain reaction. Immunological parameters were determined by immunoenzyme (immunoglobulins A, M and G) and immunoturbidimetric (haptoglobin, ceruloplasmin and α-1-antitrypsin) methods. Results. The study of the distribution of heterozygous genotypes 1772C/T HIF-1A and -634G/C VEGFA did not reveal statistically significant differences between the miners with dust lung pathology and healthy individuals in the South of the Kemerovo region. However, the study results showed heterozygous polymorphisms HIF-1A and VEGFA, particularly the level of haptoglobin α-1-antitrypsin, ceruloplasmin, and IgM, to affect the mmune status of the miners with dust lung pathology. Conclusion. Heterozygous polymorphisms 1772C/T HIF-1A and -634G/C VEGFA can be used to determine the immune response and predict the development of dust lung pathology in miners, as well as the choice of treatment and preventive measures.