Polymorphism of Ghrelin Gene in Twelve Chinese Indigenous Chicken Breeds and Its Relationship with Chicken Growth Traits

C C Li,X Guan,B Liu,D L Mo,K Li,R F Xu,T A Xiong,S L Ji,Y Z Qiangba,M J Zhu,B Fan,J Li,X H Tang,G H Chen

doi:10.5713/ajas.2006.153

Abstract

A 2,656 bp fragment of chicken ghrelin gene was cloned and SNPs were detected by PCR-RFLP and Allele Specific PCR (ASP) in 12 Chinese indigenous chicken breeds and a commercial chicken population. The results showed that there were 23 base variations and an amino acid change (Gln→Arg) in cloned chicken ghrelin gene. Three SNPs were confirmed in 13 populations and associations between this gene and growth traits of Tibetan chicken (TC) and Recessive White chicken (RW) were investigated. The results of haplotype analysis revealed that 26 haplotype genotypes were composed of eight haplotypes. The results of x 2 tests indicated that there were significant differences between genotypes or haplotype genotype frequencies in some of the breeds or sexes at 0.05 or 0.01 levels. The results of ANOVA revealed that there were significant differences between genotypes or haplotype genotypes on some growth traits of TC and RW chicken breeds at 0.05 or 0.01 levels. Multiple comparisons showed that there were significant associations between genotype CT at site 71 and some growth traits of two chicken breeds and between genotype AG at site 1,215 and body weight at 16 wk of two chicken breeds, and there was a significant association between haplotype genotype CAA/CAG and body weight and shank girth at 16 wk of two chicken breeds. (Asian-Aust. J. Anim. Sci. 2006. Vol 19, No. 2 : 153-159)

Highlights

The results showed that there were 23 base variations and an amino acid change (Gln→Arg) in cloned chicken ghrelin gene
An endogenous ligand for growth-hormone secretagogues ghrelin gene had influence on chicken hormone levels, receptor (GHS-R). This ligand, which mainly expresses in physiological circumstances and growth and development, mammal stomach and can stimulate pituitary previous studies were focused on the aspects of biology and for releasing growth hormone (GH), has a strong effect on biochemistry without exception but not the aspects of function and metabolism of stomach, gut and heart
The results of ANOVA confirm that there were significant associations between three factors and growth traits of Tibetan chicken (TC) and Recessive White chicken (RW) chicken breeds, but there were no significant associations between their interaction effects and those traits

Summary

INTRODUCTION

Kojima et al (1999) firstly purified and identified the hormone releasing factor in different doses inhibited food coding protein of ghrelin gene (GHR) in rat stomach. It is intake in neonatal chicks in the different degree. Et al, 1999; Ahmed et al, 2002; Noritoshi et al, 2003) This Both Richards (2003) and Kaiya (2004) reported that hormone increases food intake and accelerates growth rate there were four bases variations in chicken ghrelin gene at (Guido et al, 2002; Iglesias et al, 2004; Pecker et al, 2004).

MATERIALS AND METHODS

RESULTS

DISCUSSION