Polymorphism of exon 2-3 of bovine major histocompatibility complex class I BoLa-A gene

Bharat Bhushan,Pushpendra Kumar,Satyabarta Dandapat,Triveni Dutt,Shiv Pal Singh Ahlawat,Arjava Sharma,Pranab Jyoti Das,Umang Umang,Biswa Nath Patra

doi:10.1590/s1415-47572007000400009

Abstract

The exon 2-3 region of bovine major histocompatibility complex (MHC) class I BoLa-A gene was investigated for polymorphisms in three breeds of cattle originated in the Indian subcontinent namely Sahiwal, Tharparkar, Hariana, as well as crossbred (Bos taurus x Bos indicus) cattle and Jersey, the exotic breed (Bos taurus). The PCR amplified fragment of 714 bp showed distinct DdeI-, TaqI- and HinfI- RFLP patterns, thus confirming a higher degree of polymorphism in this region. To our knowledge this is the first report of HinfI restriction patterns for BoLa-A exon 2-3. The sequencing results revealed a number of nucleotide substitutions in this region, which resulted in amino acid changes. The present investigation confirmed that MHC class I BoLa-A exon 2-3 is highly polymorphic in cattle.

Highlights

The major histocompatibility complex (MHC) is a chromosomal region consisting of a series of closely linked loci or so called gene families
The MHC of cattle is known as bovine lymphocyte antigen (BoLa) located on chromosome 23
Analysis of MHC class II polymorphism has benefited from the development of PCR-RFLP methods, which have improved the ease and reliability of class II typing

Summary

Introduction

The major histocompatibility complex (MHC) is a chromosomal region consisting of a series of closely linked loci or so called gene families. Ellis et al (1998) described the DNA based typing of BoLa class I gene using sequence specific amplification of expressed class I alleles from cDNA for a few European haplotypes.

Results

Conclusion