Polymorphism of CLPG gene in three sheep breeds grown in the steppe zone of the Russian Federation.

Natalia Mosolova,Ivan Gorlov,Anatoli Kolosov,Yuri Kolosov,Nadezhda Shirokova,Elena Anisimova,Marina Slozhenkina,Viktor Ponomariov,Lyubov Getmantseva

doi:10.5455/javar.2020.g392

Abstract

Objective:This study aims to investigate the callipyge gene (CLPG) polymorphism in sheep of Edilbay, Volgograd, and Kalmyk breeds.Materials and Methods:The analysis was performed by the polymerase chain reaction–restriction fragment length polymorphisms method. The objects of the study were Edilbay fat-tailed sheep (n = 500) at the breeding plant Volgograd-Edilbay (Volgograd region), Volgograd fine-wool sheep (n = 500) at the breeding plant Romashkovskiy (Volgograd region), and Kalmyk fat-tailed sheep (n = 500) at the breeding plant Kirovsky (the Republic of Kalmykia, Yashkul rayon). To conduct the research, tissue samples of 1 cm² from sheep of Kalmyk and Edilbay breeds were taken from the auricle.Results:The allelic CLPG gene variants have been determined and genotypes of representative sampling of the three breeds of livestock grown in the steppe zone of Russia. The presented results of the CLPG gene polymorphism in these sheep breeds grown in Russia were obtained for the first time. The research study has revealed that in terms of the CLPG gene, the Edilbay, Volgograd, and Kalmyk sheep breeds have only a homozygous form.Conclusion:The results obtained expand the current understanding of the molecular markers that characterize the meat qualities of sheep.

Highlights

In the selection of farm animals, new and traditional methods of evaluation and selection are applied [1,2]
This study aims to investigate the callipyge gene (CLPG) polymorphism in sheep of Edilbay, Volgograd, and Kalmyk breeds
The presented results of the CLPG gene polymorphism in these sheep breeds grown in Russia were obtained for the first time

Summary

Introduction

In the selection of farm animals, new and traditional methods of evaluation and selection are applied [1,2]. Some desirable commercially important traits and properties of meat quality were manifested, i.e., a higher percentage of meat yield, a larger loin and leaner meat, limbs were higher appreciated In these lambs, the best qualities of carcass were expressed in better yield (compared to lambs of ordinary muscularity) of whole limbs, sirloin part, loin on the bone and shoulder by 11.8%, 4.7%, 2.5%, and 2.3%, respectively [12]. The best qualities of carcass were expressed in better yield (compared to lambs of ordinary muscularity) of whole limbs, sirloin part, loin on the bone and shoulder by 11.8%, 4.7%, 2.5%, and 2.3%, respectively [12] In this regard, the purpose of the work was to study the Callipigia gene polymorphism that affects the manifestation of muscular hypertrophy in the pelvic and posterior areas in sheep of Edilbay, Volgograd, and Kalmyk breeds. In Russia, the polymorphism of this kind has not been studied in the Edilbay, Volgograd, and Kalmyk sheep

Ethical approval

Results and Discussion

Conclusion

Conflict of interests