Abstract
Abstract. This study covered a population of 91 cows aged 3–6 years. Enzootic bovine leukaemia (EBL) was diagnosed with ELISA and PCR tests. The assays were performed in the 1st, 2nd, and 3rd month after calving. Use was made of anti-bovine monoclonal antibodies (Mab), (VMDR Inc. Pullman-USA) and conjugates of FITC and R-PE (Medac – Germany) with an indirect immunofluorescence reaction (IMF). A differentiation observed between the population numbers of CD19 + B lymphocytes and CD2 + T lymphocytes as well as the CD8 + T lymphocyte sub-population was found to be significant between cows with different genotypes of AcP. Moreover, a correlation was found between the polymorphism of AcP and EBL incidence in cows with reference to the number and percentage of CD19 + B lymphocytes (AcP polymorphism x EBL interaction). A significant differentiation in the profile of peripheral blood lymphocytes was observed between EBL-positive and EBL-negative cows as well as over the first three months after calving. The results reported in this study seem to indicate the potential contribution of a genetic predisposition connected with the expression of biological functions of the blood leukocyte acid phosphatase system in the activation and proliferation of these cells.
Highlights
Acid phosphatase (AcP) (EC 3.1.3.2) of blood leukocytes constitutes an important element of an enzymatic equipment of grain-like structures of lysosome cells of the blood leukocyte system
The aim of the study was to determine the relationship between a monogenic system of blood leukocyte acid phosphatase and the differentiation of the population sizes of CD19+ B lymphocytes and CD2+ T cells as well as the differentiation of T - cytotoxic (CD8+) and T - helper lymphocytes (CD4+) subsets in the first months of lactation of cows naturally-infected with bovine leukaemia virus
A co-operate was observed between AcP polymorphism and enzootic bovine leukaemia (EBL) incidence with respect to the number and percentage of CD19+ B lymphocytes
Summary
Acid phosphatase (AcP) (EC 3.1.3.2) of blood leukocytes constitutes an important element of an enzymatic equipment of grain-like structures of lysosome cells of the blood leukocyte system. The dominant gene AcPB controls the synthesis of B isoenzyme present in AB phenotype This phenotype was determined by two genotype groups: dominant homozygote B/B and heterozygote B/b. The recessive gene AcPb in homozygous genotype determines phenotype A, in which isoenzyme B does not occur. Fraction A, which is encoded independently of a genetically-determined acid phosphatase polymorphism, occurs in both phenotype groups (KACZMARCZYK and WALAWSKI, 1992). The AcP polymorphism demonstrates associations with blood leukocyte morphological differentiation and granulocyte metabolic efficiency (KACZMARCZYK et al, 1989; KACZMARCZYK and TAUBE, 1990). These relationships seem to indicate the possible participation of AcP in natural defense mechanisms against pathogens (KACZMARCZYK et al, 1999). A significant role in the organic defense against BLV has been ascribed to BoLA class II genes, mainly of locus DRB3.2
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