Polymorphism in the SCD gene is associated with meat quality and fatty acid composition in Iranian fat- and thin-tailed sheep breeds

Abstract

The enzyme stearoyl-coenzyme A-desaturase (SCD) has been proposed as a key regulator for fat content and fatty acid composition of meat. In this study, a DNA sequence and single-strand confirmation polymorphism analysis were performed in an attempt to investigate the relationship between variation within the SCD gene and fatty acid profiles, physicochemical composition, and the quality characteristics of longissimus dorsi (LD) muscle in two traditional fat- (Chall) and thin- (Zel) tailed Iranian sheep breeds. Only one previously reported SNP (g.379A>T) located in the 5′ untranslated region of the SCD gene was observed. Two previously reported allelic variants, SCD-1 and SCD-2, constituted two previously reported genotypes, “A” and “B”. Based on t-test, no allelic and genotypic frequency differences were observed between Chall and Zel breeds. Lambs with genotype “B” significantly had lower total saturated fatty acid (SFA) level (p<0.05) and more linoleic (p<0.02), arachidonic (p<0.002), and eicosapentaenoic (EPA) (p<0.001) acids and total poly-unsaturated fatty acid (PUFA) (p=0.002) contents and PUFA: SFA ratio (p<0.004) than those with genotype “A”. Meat color of the genotype “B” lambs was lower for a* (p<0.05) and higher for L* (p<0.08) compared to the genotype “A” ones. There were some interactions between breed and SCD gene in terms of palmitic acid (p<0.04), cooking loss (p<0.05), and protein content (p<0.03) of LD muscle. SCD locus explained 17.3%, 15.3%, 13%, and 12% of the phenotypic variation in terms of EPA and arachidonic acid, and PUFA contents and PUFA: SFA ratio, respectively. Interestingly, the results of the correlation analyses showed the significant negative correlations between healthy fatty acid indices (PUFA and PUFA: SFA) and meat color measures of a* and b* (r=−0.26 to r=−0.40, p<0.05) and the significant positive correlations between n–6: n–3 PUFA, one of the main harmful fatty acid indices, and a* (r=0.41, p<0.01) and b* (r=0.27, p<0.05) values which are coordinated with the effect of SCD genotypes on fatty acid composition and color of meat. So, both fatty acid composition and meat color traits should be considered in marker-assisted selection based on SCD gene. Overall, it seems that the genotype “B” of SCD gene is a more desirable genotype when selecting lambs in terms of both fatty acid composition and color of meat, because selecting this genotype would result in higher proportion of healthy fatty acids, lower proportion of harmful fatty acids, and better meat color.