Abstract
In the article, the results on the development of cell selection scheme for sugar beet genotypes resistant to high temperature are presented. In terms of increase in the callus diameter and the ability to regenerate plants, lines of high-temperature resistant genotypes Yaltushkivskyi MS 72, Ukrainian MS 70, Ukrainian MS 72 and Katiusha found to be promising for breeding. Experiments confirmed that 5-7 times increased proline content is typical of the temperature resistant lines. We investigated the molecular and genetic polymorphism in sugar beet genotypes using RAPD and SSR analyses. Fourteen alleles were identified and analyzed by four RAPD markers and five alleles by GZM 086 marker. Cluster analysis using DNA markers shows that Ukrainian MS 72 and Ivanivskyi MS 33 are the most similar genotypes, and they are found in the same cluster. It was determined that the temperature resistant sugar beet genotypes Yaltushkivskyi MS 72, Ukrainian MS 70, Ukrainian MS 72 and Katiusha are genetically distant and therefore can be used to create heterotic hybrids.
Highlights
One of the most important ways to increase the productivity of sugar beet (Beta vulgaris L.), which provides a significant contribution to the Ukrainian and world economies, is the creation of new hybrids of optimal response to environmental changes that can be adjusted by a cultivation technology and genetically determined resistance to abiotic factors at certain ontogenetic stages (Roik et al, 2010; Sytnik, 2007; Husiev, 2011; Loel and Hoffmann, 2014)
The purpose of this study was to determine the polymorphism in different sugar beet genotypes in the process of developing technologies of cell selection in vitro in order to create a promising material with signs of temperature resistance
To create temperature resistant lines, the obtained calluses of different sugar beet genotypes were exposed to high temperature
Summary
One of the most important ways to increase the productivity of sugar beet (Beta vulgaris L.), which provides a significant contribution to the Ukrainian and world economies, is the creation of new hybrids of optimal response to environmental changes that can be adjusted by a cultivation technology and genetically determined resistance to abiotic factors at certain ontogenetic stages (Roik et al, 2010; Sytnik, 2007; Husiev, 2011; Loel and Hoffmann, 2014). The purpose of this study was to determine the polymorphism in different sugar beet genotypes in the process of developing technologies of cell selection in vitro in order to create a promising material with signs of temperature resistance
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