Abstract
The role played by hereditary factors in the development of diabetes mellitus type 2 (DM2) has not yet been fully established. Therefore, the purpose of our study was to investigate the prevalence of adiponectin and polymorphism in its gene receptors in connection with the primary symptoms of DM2 pathogenesis. Genomic DNA was isolated from the whole blood of 94 patients with an established diagnosis of DM2 using the phenol–chloroform method. Gene polymorphisms were determined using real-time polymerase chain reaction (PCR). The most common polymorphic variants in patients with DM2 were the genotypes AA (rs11061971) and GG (rs16928751) on the ADIPOR2 gene. A strong correlation was found between the rs16928751 polymorphism on the ADIPOR2 gene and increased body mass index (BMI). TG (rs2275737) ADIPOR1 gene genotype carriers were found to have the highest levels of glycosylated hemoglobin (HbA1), whereas TT (rs2275738) caused stable hyperglycemia. In addition, the rs16928751 ADIPOR2 gene polymorphism showed an association with the development of key mechanisms of DM2 in the Russian population, although a number of genomic searches failed to show any association of this gene with DM2. Unique gene variants associated with the risk of developing DM2 in the Crimean population were established.
Highlights
In the pathogenesis that leads to type 2 diabetes mellitus (DM2), both hereditary predisposition and obesity as the result of lifestyle and nutrition habits are considered key players
We have studied the polymorphisms of the ADIPOQ gene + 45 T/G and
We found a relationship between the ADIPOR2 gene rs16928751 polymorphism and high body mass index (BMI) index values
Summary
In the pathogenesis that leads to type 2 diabetes mellitus (DM2), both hereditary predisposition and obesity as the result of lifestyle and nutrition habits are considered key players. For this reason, investigations of polymorphism in candidate genes involved in DM2 pathogenesis are of great practical importance. The adiponectin-mediated pathway, among others, is associated with impaired glucose tolerance, insulin resistance (IR), and obesity [1]. Adiponectin is encoded by a specific gene (ADIPOQ) and synthesized by white adipose tissue cells. A low concentration of adiponectin in the blood, typical for DM2 patients, is associated with a decrease in lipid oxidation, increased triglyceride concentrations, and compromised glucose consumption in peripheral tissues [2]
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