Polymorphism identification in the goat &amp;lt;i&amp;gt;THRSP&amp;lt;/i&amp;gt; gene and association analysis with growth traits

X An,B Cao,J Peng,H Zhao,L Bai,Y Song,J Wang,J Hou

doi:10.5194/aab-55-78-2012

Abstract

Abstract. cIn this study, we reported the analysis of THRSP gene polymorphisms in 610 goats of three breeds: Xinong Saanen (SN), Guanzhong (GZ) and Boer (BG). We identified new allelic variant: P2-G39294A (GenBank acc. no. JN618075) in the three goat breeds. At P2 locus, GG, GA and AA genotypes were found in the three goat breeds. The frequencies of G allele were 0.54–0.55 and frequencies of A allele were 0.46–0.45, and the PIC was 0.37. The SNP locus was in Hardy-Weinberg disequilibrium in Boer goat breed (P<0.05). Association of polymorphisms with growth traits was done at P2 locus in Boer goat breed. The result showed that AA genotype had remarkable growth traits at P2 locus (P<0.05). Therefore, these results suggest that THRSP gene is a strong candidate gene that affects growth traits in goat.

Highlights

The physiological regulation of growth traits is under the control of multiple genes, which may be important candidates for unraveling the genetic variation in economically relevant traits in farm animals (Wu et al 2008, An et al 2010)
The P2 locus were in Hardy-Weinberg equilibrium in SN and GZ breeds (P>0.05), which showed the genotypic frequencies has not been affected by selection, mutation or migration in both goat breeds
In BG breed, the P2 locus were in Hardy-Weinberg disequilibrium (P

Summary

Introduction

The physiological regulation of growth traits is under the control of multiple genes, which may be important candidates for unraveling the genetic variation in economically relevant traits in farm animals (Wu et al 2008, An et al 2010). Thyroid hormone responsive (THRSP) gene encodes a small acidic protein expressed predominately in the lactating mammary gland, fat, and liver (Zhan et al 2006). Invernizzi et al (2010) reported that expression of THRSP decreased by day 7 with both diets (saturated lipid and a blend of fish/soybean oil) and returned to basal levels by day 21 in bovine mammary tissue. THRSP is an important transcription factor that controls the expression of several lipogenic genes (Towle et al 1997).

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