Abstract
We developed twenty polymorphic microsatellite markers for the long-spined sea urchin Diadema setosum by screening microsatellite enriched library, and characterized their polymorphism using two populations of 45 individuals. The number of alleles per locus ranged from 4 to 17, and the observed and expected heterozygosity varied from 0.0714 to 1.000 and 0.6655 to 0.9321, respectively. Twelve loci conformed to the Hardy-Weinberg equilibrium after Bonferroni correction. These polymorphic microsatellite markers will facilitate studies of population genetics and connectivity in D. setosum.
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