Polymorphic heterologous microsatellite loci for population genetics studies of the white-faced ibis Plegadis chihi (Vieillot, 1817) (Pelecaniformes, Threskiornithidae)

Andiara Silos Moraes De Castro E Souza Souza,Silvia Nassif Del Lama,Carolina Isabel Miño

doi:10.1590/s1415-47572012005000018

Andiara Silos Moraes De Castro E Souza Souza, Silvia Nassif Del Lama + Show 1 more

Open Access

https://doi.org/10.1590/s1415-47572012005000018

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Abstract

We screened 44 heterologous microsatellites isolated in species of the families Threskiornithidae, Ciconiidae and Ardeidae for their use in a migratory waterbird, the white-faced ibis Plegadis chihi (Vieillot, 1817) (Threskiornithidae). Of the screened loci, 57% amplified successfully and 24% were polymorphic. In two breeding colonies from southern Brazil (N = 131) we detected 32 alleles (2-10 alleles/locus). Average He over all loci and colonies was 0.55, and the combined probability of excluding false parents, 98%. There was no departure from HWE in any loci or population. Eru6 and Eru4 loci were in non-random association in the Alvorada colony, and NnNF5 and Eru5 in both populations. AMOVA analysis indicated that most of the genetic diversity was contained within populations. Structure analysis suggested a single population, and F(ST) value showed weak genetic structuring (F(ST) = 0.009, p = 0.05). The two populations are apparently connected through gene-flow. The panel of six microsatellites optimized here was sufficiently informative for characterizing the genetic diversity and structure in these natural populations of the white-faced ibis. The information generated could be useful in future studies of genetic diversity, relatedness and the mating system in Plegadis chihi and related species.

Highlights

The white-faced ibis Plegadis chihi (Vieillot, 1817) (Pelecaniformes, Threskiornithidae) is a migratory longlegged wading bird, with a long, downward-curved bill and attractive metallic bronze plumage (Dark-Smiley and Keinath, 2003)
Further information is provided on population genetic parameters and patterns of genetic structuring in samples of two breeding colonies located in the extreme geographic distribution of this species in south Brazil
Except for loci isolated in P. ajaja and M. americana (HEX-labeled), all the primers were dynamically labeled with HEX, FAM or TET, using M13 methodology (Schuelke, 2000)

Summary

Introduction

The white-faced ibis Plegadis chihi (Vieillot, 1817) (Pelecaniformes, Threskiornithidae) is a migratory longlegged wading bird, with a long, downward-curved bill and attractive metallic bronze plumage (Dark-Smiley and Keinath, 2003). This lack of species-specific microsatellite markers is one of the reasons why, in populations of the white-faced ibis, nuclear neutral genetic variability remains neglected.

Results

Conclusion