Abstract
In the present study, distinct Chromobacterium violaceum strains were genetically characterized from polymorphicDNA fi ngerprinting patterns. Such assays were performed in presence of primer ERICII,originally designed from enterobacterial intergenic repetitive element sequences. Clustering analysis ofthe ERIC-PCR products and genetic similarity phenogram were estimated by using the coeffi cient ofSorensen-Dice and the UPGMA algorithm in the NTSYS-pc computer program. Genotypic diversityand the Shannon gene diversity indexes among the bacterial strains were calculated using the populationgenetics package POPGENE. Four main groups including individuals belonged to specifi c strainswere defi ned by clustering analysis of ERIC-PCR products, in which similarity coeffi cients varied from0.57 to 1 The inter-strain genetic distance and identity calculations revealed that the largest distancewas observed between C. violaceum ATCC12472 and 07.1 strains (0.6414) and that the greatest identitybetween those identifi ed as C. violaceum ATCC12472 and CBM strains (0.7622), confi rming the patterngenerated in the similarity phenogram. Considering the accentuated reproducibility in amplifi cation reactions and the highly polymorphic DNA fi ngerprinting profi le observed for each bacterial strain,these fi ndings create real possibilities for the detection of C. violaceum strains in microbial mixed culturesthrough ERIC-PCR assays.
Highlights
The Chromobacterium violaceum is a free-living bacterium commonly found in the soil and in the water from tropical and subtropical regions around the world
We characterized genetically distinct C. violaceum strains based on their polymorphic DNA fingerprinting patterns
The eletrophoretical profile generated from enterobacterial repetitive intergenic consensus (ERIC)-PCR products in the ATCC12472 and CBMA.1 strains yielded multiple distinct DNA fragments of sizes ranging from 100 to 3,000 bp and the number of amplified bands was corresponding to 17 bands per individual
Summary
The Chromobacterium violaceum is a free-living bacterium commonly found in the soil and in the water from tropical and subtropical regions around the world. The 124 to 127 bp enterobacterial repetitive intergenic consensus (ERIC) elements, otherwise known as intergenic repeat units, have been discovered in the genomes of the aforementioned organisms as well as other gram negative species and are present at approximately 30 to 150 copies [5, 6]. The function of these highly repeated and conserved elements is not yet well elucidated, several evidences have suggested their involvement in stabilizing mRNA, in translational coupling between genes, in homologous recombination, in chromosomal explain its DNA sequence conservation and ubiquitous distribution. Amplification-based DNA fingerprinting techniques have been used as a powerful tool in the molecular genetic analysis of the genetic biodiversity in a great variety of prokaryotic organisms [4, 8]
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