Polymethyl methacrylate resin for provisional restoration affects rat macrophage function in in vitro conditions.

Abstract

This study evaluated the cytotoxic effects of polymethyl methacrylate resin extracts on rat macrophage viability in in vitro conditions. Prepared test specimens were immersed in 5 mL of artificial saliva and incubated for 24, 48, and 72 h at 37°C. The cytotoxicity of the obtained solutions of extracted resins, used as a stock solution (100%) and diluted with Roswell Park Memorial Institute (RPMI) medium to obtain the working solutions (50, 40, 30, 20, 10, and 5%), was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. No dose-dependent toxic activity in macrophage culture was detected for the three types of extracts obtained after 24, 48, and 72 h of material extraction. The shortest extraction of material was found to be completely non-toxic, and the 20% concentration of this extract obtained caused a significant increase in cell ability to metabolize MTT. Extracts obtained after 72 h of extraction showed the highest cytotoxic potential of 50%, 40% and 30%, and extracts obtained after 48 and 72 h of extraction at concentrations of 5% and 10% had a proliferative effect on the macrophage cell line. This study demonstrated that the highest cytotoxic effect was observed in cells exposed to the highest concentrations (50, 40, and 30%) of the extracts that were extracted for 72 h.