Abstract
Abstract Polymer-supported enzymic oligosaccharide synthesis may lead to products of biological interest otherwise not readily attainable. Thanks to the “polymer handle”, the products are readily isolated, purified and characterized. This makes it possible to deal efficiently with comparative acceptor specificity and demonstrate de novo synthesis: the specific example of glycogen synthetase is discussed. The derivatised polymers used were crosslinked polyacrylamide gel beads, water-soluble poly(vinyl alcohol) and water-soluble polyacrylamide. The saccharide products were removed from the polymer supports by photolysis of substituted o-nitrobenzyl linkages or by enzymic hydrolysis using a α-chymotrypsin.
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