Abstract
This work describes the preparation of polymeric non-viral system for transport and delivery of nucleic acids. An amphiphilic poly(2-(dimethylamino)ethyl methacrylate)-block-poly(e-caprolactone)-block-poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA20-b-PCL70-b-PDMAEMA20) triblock copolymer was synthesized and used for formation of cationic micelles and subsequent complexation with DNA. Next, a protective polymer coating on the complex surface and removal of copolymer from the particle interior were conducted. In this way, polymer nanocapsules containing DNA molecules were obtained. The synthesized polymer, cationic micelles, complexes, and polymer capsules were investigated by proton nuclear magnetic resonance, gel permeation chromatography, dynamic and electrophoretic light scattering, and transmission electron microscopy. In vitro cytotoxicity assessment of the different systems revealed very good tolerance to human cells.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
