Polymeric Network of Amines and Metals Colloids: Conformational Analysis of Interaction, Orientation with DNA

Abstract

Over the last decade numerous promising synthetic, non-viral gene delivery systems have been developed and an outline of their potential advantages and disadvantages has materialized [1]. Synthetic vectors have advantages in interconnected pharmaceutical issues, safety, and ease of use but tend to be less efficient than some viral systems. Advanced delivery systems can be developed that will have higher potential and more advantages. Moreover, physicochemical properties including charge, dissociation behavior, and the shape and nanoparticle size of the cationic complexes can play a significant role in efficient gene transfer in vitro and in vivo. Even though the interaction between the polymeric cations and DNA is electrostatic in origin, geometric and chemical structures of the polymer also play a substantial character in complex formation. Even the number of positive charges and the spacing of cationic charges within the polymer upsets the size of DNA nanoparticles fashioned by polycations [2]. Hence, a complete set of biophysical measurements for the characterization of physical and chemical properties of cationic complexes offered consists of cationic polymers/surfactants with DNA. By considering this beginning as a study on the structural properties of the cationic complexes, the understanding of the effect of polymer architecture on the DNA/cationic polymer interaction at the molecular level in terms of DNA stability, conformation, size, shape, binding affinity, and complete thermodynamics of the interaction are also necessarily required [3].