Abstract
Septicemia epizootica (SE) is a common fatal systemic disease in cattle and buffalo due to Pasteurella multocida serotype B:2 in South and Southeast Asia countries, including Indonesia. This infectious agent is generally considered an opportunistic pathogen and located in the nasopharynx of the animal. To support the disease’s diagnosis, an acceptable identification procedure must be established. This study was to confirm bovine P. multocida isolate that has been identified through biochemical approaches at the first step, with the Polymerase Chain Reaction method. The isolate was obtained from the fatal outbreak of cattle in Kupang in 2016 and subjected to be identified using biochemical characterization, but it was time consumed. The API 20NE was applied to identify the isolate and help to save time. The PCR result showed positive for 16 sRNA and kmt genes, both were classified as specific genes, and the capsular serotype was detected in less than 24 hours. It indicates that PCR confirms biochemical technique, and it is an appropriate and faster method in detecting pathogen agents than biochemical one.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: IOP Conference Series: Earth and Environmental Science
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
