Abstract
Three sets of polymerase chain reaction (PCR) primers for Trichinella isolates were constructed by the following method. Random amplified polymorphic DNA (RAPD) of the corresponding isolates was produced and several fragments were sequenced. Primer sets SB4-2 and SB4-4 were designed from the resulting sequence that could amplify exclusively DNA from T. spiralis. These primers produced extra fragments in addition to the expected fragment, and the banding pattern differed among some strains of T. spiralis suggesting that the pattern can serve as a gene fingerprint of T. spiralis. Primer pairs SB5 amplified exclusively DNA from T. pseudospiralis. 1 1 The accession numbers for sequences SB4 and SB5 are U42045 and U37588, respectively.
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