Abstract
Copyright information: Taken from Extracellular mitochondrial DNA and oxidatively damaged DNA in synovial fluid of patients with rheumatoid arthritisArthritis Research & Therapy 2003;5(5):R234-R240.Published online 25 Jun 2003http://www.ncbi.nlm.nih.gov/pmc/articles/PMC193725.Copyright © 2003 Hajizadeh et al., licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL. SDS–polyacrylamide-gel electrophoresis of PCR-amplified mitochondrial DNA (mtDNA) fragments (453 bp) of plasma and SF of five patients with RA. A semi-quantitative method was used to assign the PCR product intensities to positive or negative categories. An arbitrary cutoff point was assigned that was relative to the intensity of the 676-bp band (arrow) of the molecular mass marker on the gel. Lane 1, molecular weight marker; lane 2, water only (control); lane 3, plasma from patient 1 (plasma-1); lane 4, SF from patient 1 (SF-1); lane 5, plasma-2; lane 6, SF-2; lane 7, plasma-3; lane 8, SF-3; lane 9, plasma-4; lane 10, SF-4. The samples in lanes 11 (plasma-5) and 12 (SF-5) were spiked with 10 ng of purified mtDNA.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
