Polymerase chain reaction based epidemiological investigation of canine parvoviral disease in dogs at Bareilly region

Jobin Thomas,Surendra Kumar Badasara,T K Goswami,Suman Verma,Mithilesh Singh

doi:10.14202/vetworld.2014.929-932

Jobin Thomas, Surendra Kumar Badasara + Show 3 more

Open Access

https://doi.org/10.14202/vetworld.2014.929-932

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Journal: Veterinary world	Publication Date: Nov 1, 2014
Citations: 18	License type: cc-by

Affiliation: Indian Veterinary Research Institute

Abstract

Aim: The aim of this study was to screen the suspected samples by polymerase chain reaction (PCR) and epidemiological analysis of positive cases of canine parvovirus type2. Materials and Methods: Fecal samples were collected from dogs suspected for canine parvovirus type 2 (CPV-2) and viral DNA was extracted. Primers were designed, and PCR was done with all extracted DNA samples. Age, sex and breed wise distribution of positive cases were analyzed. Results: Out of a total 44 collected fecal samples, 23 were found to be positive for CPV-2 by developed PCR. The disease was found to be more common in Labrador male pups of 3-6 months of age. The percentage of positive cases in vaccinated dogs was found to be around 17.4%. Conclusion: Almost half (52.3%) of total collected samples were found to be positive by PCR. However, number of field samples are needed to further validate this test and additionally sequence analysis needs to be done to ensure the prevalent field strain of CPV-2.

Highlights

Canine parvovirus type 2 (CPV-2) is a non-enveloped single-stranded DNA virus which is responsible for an acute and sometimes fatal enteritis in dogs [1]
Almost half (52.3%) of total collected samples were found to be positive by polymerase chain reaction (PCR)
Intestinal form is more common which is characterized by hemorrhagic gastroenteritis, and the less common myocardial form is characterized by myocarditis [5]

Summary

Introduction

Canine parvovirus type 2 (CPV-2) is a non-enveloped single-stranded DNA virus which is responsible for an acute and sometimes fatal enteritis in dogs [1]. The virus first appeared in 1978, probably arose from a very closely related virus in cats, the feline panleukopenia virus through a small number of mutations in the single capsid protein. It has spread worldwide and being replaced by some new antigenic variants likes CPV-2a, CPV-2b and CPV-2c [3]. This is one of the important pathogenic viruses affecting dogs with high morbidity and mortality [4]. Parvoviral disease of dogs is mainly manifested as two forms, intestinal and myocardial form. Vaccination is the most efficient strategy for the control of the disease

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