Abstract
We have used a set of microsatellite polymorphisms (MSPs) to examine the location and frequency of allele loss throughout the genome in a panel of 25 human epithelial ovarian tumours. When more than one MSP was employed per arm, mean informativity was 85.2% (range 64-100%). The average fractional allelic loss was 0.28 (range 0-0.65). A high frequency of allele loss was seen at 5q (40%), 9q (48%), 11p (43%), 14q (46%), 15q (40%), 17p (61%), 17q (64%), 19p (45%) and Xp (40%), confirming previous findings at some sites, but also suggesting the existence of new tumour-suppressor genes in regions (9q, 14q, 15q) which have not previously been studied in ovarian cancer. For 9q and 14q, partial loss of the arm was more common than loss of heterozygosity for all loci. There was a significant relationship between allele loss affecting the short arm of chromosome 17 and allele loss affecting 17q (P < 0.001). No other relationship was detected between allele losses at different sites. Polymerase chain reaction allelotyping is suitable for the examination of very small tumour samples and tumours in which classical karyotyping is problematic.
Highlights
When more than one microsatellite polymorphisms (MSPs) was employed per arm, informativeness increased to a mean of 85.2%
There was a significant relationship between allele loss affecting the short arm of chromosome 17 and allele loss affecting 17q (P
No other relationship was detected between allele losses at different sites in this cohort of tumours
Summary
Locus PNAS 1983-80-6932 AJHG 1989-44-388 S 1992-258-67 HMG 1992-1-137 I 1989-30-393 S 1992-258-67 CCG 1989-52-68 S 1992-258-67 G 1992-14-209 AJHG 1991-49-621 G 1992-14-209 G 1992-14-209 NAR 1991-19-5794 CCG 1991-58-284 AJHG 1988-43-638 CCG 1988-48-25 S 1992-258-67 CCG 1985-40-696 CCG 1991-58-323 G 1991-11-737 S 1989-245-1059 S 1989-245-1059 CCG 1993-63-45 S 1992-258-67 N 1990-344-36 NAR 1991-19-5093 G 1992-12-607 G 1992-14-715 G 1992-14-715 G 1992-14-715 G 1992-14-715 G 1992-12-604 G 1992-12-604 NAR 1992-20-1431 S 1992-258-67 S 1992-258-67 NAR 1991-19-4308 S 1992-258-67 S 1985-228-1401 S 1992-258-67. MSPs are listed by their official locus name ('D number'), chromosomal location and oligonucleotide primer sequences. No tumour sample contained more than 40% normal tissue. Oligonucleotides were obtained from the HGMP Resource Centre (Harrow, UK), or were synthesised locally.
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