Polymer Optical Waveguide Grating-Based Biosensor to Detect Effective Drug Concentrations of Ginkgolide A for Inhibition of PMVEC Apoptosis.

Chunxue Wang,Bendong Fu,Pengfei Yi,Haibing Dong,Jiang Li,Daming Zhang,Changming Chen,Haiqing Shen

doi:10.3390/bios11080264

Abstract

In this work, we successfully developed a fluorinated cross-linked polymer Bragg waveguide grating-based optical biosensor to detect effective drug concentrations of ginkgolide A for the inhibition of pulmonary microvascular endothelial cell (PMVEC) apoptosis. Fluorinated photosensitive polymer SU-8 (FSU-8) as the sensing core layer and polymethyl methacrylate (PMMA) as the sensing window cladding were synthesized. The effective drug concentration range (5–10 µg/mL) of ginkgolide A for inhibition of PMVEC apoptosis was analyzed and obtained by pharmacological studies. The structure of the device was optimized to be designed and fabricated by direct UV writing technology. The properties of the biosensor were simulated with various refractive indices of different drug concentrations. The actual sensitivity of the biosensor was measured as 1606.2 nm/RIU. The resolution and detection limit were characterized as 0.05 nm and 3 × 10−5 RIU, respectively. The technique is suitable for safe and accurate detection of effective organic drug dosages of Chinese herbal ingredients.

Highlights

It has been shown that ginkgolide A can significantly reduce pulmonary histological damage and effectively inhibit pulmonary microvascular endothelial cell (PMVEC) apoptosis, which is advantageous to reduce acute lung injury (ALI) [3]
It is obvious that hydrogen bonding forces and binding energy are stable and strong between the polymer waveguide materials and ginkgolide A, which is useful for realizing high detection sensitivity of the optical biosensor
The relationship between the sensitivity S and the limit of detection (LOD) L for the biosensor is given as L = R/S, where the sensor resolution R is related to system noise

Summary

Introduction

Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. It has been shown that ginkgolide A can significantly reduce pulmonary histological damage and effectively inhibit pulmonary microvascular endothelial cell (PMVEC) apoptosis, which is advantageous to reduce acute lung injury (ALI) [3]. In this case, it is important to precisely select effective drug concentrations of ginkgolide A in order to realize the scientific biomedical application of TCM. A fluorinated copolymer waveguide grating-based optical biosensor to detect effective drug concentrations of ginkgolide A for the inhibition of PMVEC apoptosis was designed and fabricated. The technique will be useful to ensure the effectiveness and safety of organic drug dosages efficiently and accurately

Sensing Polymer Waveguide Materials

Biosensor Design and Fabrication

Biosensor Measurement and Discussion

Findings

Conclusions