Abstract
This article evaluates several techniques for measuring nanoparticle size in suspension. The direct techniques measure the nanoparticle size by electron microscopy. However, with this technique, the particles must be observed dry, except in the case of environmental microscopy, and their distribution is calculated by performing image analysis. Other indirect methods, based on measurements of intensity fluctuations allow size measurement but these methods are dependent on sample preparation (concentration, polydispersity, etc.). Two types of materials are characterized and discussed herein: model polystyrene latex particles used for size standardization in several devices and polydisperse wheat protein nanoparticles prepared in our laboratory. Our results show that no size characterization method is totally universal and absolute. Coupling at least two different methods allows for more rigorous size characterization.
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