Abstract
Fluorescence resonance energy transfer (FRET) measurements were used to monitor polymer diffusion in poly(butyl methacrylate) latex films with a polymer molar mass of Mw ≈ 125 000 (Mw/Mn = 2.5). These experiments employed the nonfluorescent acceptor chromophore NBen, which allowed faster data acquisition at lower acceptor dye concentration (0.3, 0.5 mol %) than previous experiments with anthracene (1 mol %) as the acceptor. The data were analyzed in two distinct ways. Our traditional simplified approach involved calculating fm values for the quantum efficiencies of FRET (ΦET). Apparent diffusion coefficients Dapp were calculated by making rather severe assumptions about fm. In addition, we carried out mathematical simulations of diffusion which satisfied Fick's laws in a spherical geometry. The concentration profiles of donor and acceptor were introduced into equations that describe the rate for of energy transfer, and donor decay profiles were simulated (t). By comparing simulated and experimental decay ...
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