Abstract
Low angle laser light scattering (LALLS) and multi-angle laser light scattering (MALLS) methods have become valuable, if not indispensable, tools for the chemist and biochemist, since they can be used to determine the absolute molecular weight and size of molecules in solution. When linked to a gel permeation chromatography system, they become even more powerful tools, providing information on molecular weight averages and their distributions (polydispersity). LALLS has essenttially been superseded by the development of on-line MALLS and size-exclusion chromatography (SEC)/MALLS systems, which have facilitated the greater application of light scattering theory in structure elucidation. For example, the determination of root mean square radii, molecular conformation and structure, branching ratios etc., are now readily achievable. This paper reviews the “theoretical backbone” of light scattering methods and the technology available for use in this particular aspect of macromolecular characterisation. Particular emphasis is placed on the use of SEC/MALLS and SEC/LALLS for the molecular weight elucidation of polysaccharides (such as carrageenans, starch, hyaluronic acid and dextrans), proteins, and the examination of branching in large macromolecular structures
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