Polymer-Based Functional Cantilevers Integrated with Interdigitated Electrode Arrays-A Novel Platform for Cardiac Sensing.

Pooja P Kanade,Nomin-Erdene Oyunbaatar,Dong-Weon Lee

doi:10.3390/mi11040450

Pooja P Kanade, Nomin-Erdene Oyunbaatar + Show 1 more

Open Access

https://doi.org/10.3390/mi11040450

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Journal: Micromachines	Publication Date: Apr 24, 2020
Citations: 12	License type: CC BY 4.0

Affiliation: Chonnam National University

Abstract

Heart related ailments are some of the most common causes for death in the world, and some of the causes are cardiac toxicity due to drugs. Several platforms have been developed in this regard over the years that can measure electrical or mechanical behavior of cardiomyocytes. In this study, we have demonstrated a biomedical device that can simultaneously measure electrophysiology and contraction force of cardiomyocytes. This dual-function device is composed of a photosensitive polymer-based cantilever, with a pair of metal-based interdigitated electrodes on its surface, such that the cantilever can measure the contraction force of cardiomyocytes and the electrodes can measure the impedance between cells and substrate. The cantilever is patterned with microgrooves so that the cardiomyocytes can align to the cantilever in order to make a higher cantilever deflection in response to contraction force. Preliminary experimental results have identified the potential for use in the drug-induced cardiac toxicity tests, and further optimization is desirable to extend the technique to various bio-sensor areas.

Highlights

Heart related ailments are some of the most common causes for death in the world, and some of the causes are cardiac toxicity due to drugs
In order to measure mechanical response in the form of contraction force, micro-posts and cantilevers have been used, such that these devices would measure the amount of deflection occurring because of the cardiomyocytes and contraction force can be calculated based on this deflection [5,6,7,8]
We propose a device wherein impedance is measured with the help of Interdigitated electrode array (IDE)

Summary

Introduction

Heart related ailments are some of the most common causes for death in the world, and some of the causes are cardiac toxicity due to drugs. 90% of drugs that enter clinical trials fail to commercialize owing to their toxic side effects on the heart [2] Development of such techniques that can measure electrophysiology and contraction force of cardiomyocytes is of critical importance. To measure the contraction force, technique of measuring cantilever deflection is a better and efficient technique in which real time data and beating pattern can be obtained. ECIS is a non-invasive and label-free method and real-time information on cell behavior can be obtained. Neonatal rats’ cardiomyocytes were cultured on the device for analyzing the bending displacement of cantilever and impedance from IDE. To check the response to cardiac toxicity, two drugs—namely Verapamil and E-4031—were added and the cantilever deflection and IDE impedance was measured

Working Principle of the Device

Cell Culture

Simultaneous Measurement of Impedance and Cantilever Displacement

Results and Discussion

Conclusions