Abstract
The novelty of the study is the development, creation, and investigation of biodegradable polymeric membranes based on polylactide, that are capable of directed release of large molecular weight biomolecules, particularly, prourokinase protein (MW = 46 kDa). Prourokinase is a medication with significant thrombolytic activity. The created membranes possess the required mechanical properties (relative extension value from 2% to 10%, tensile strength from 40 to 85 MPa). The membranes are biodegradable, but in the absence of living cells in a water solution they decompose by less than 10% in half a year. The created membranes are capable of controlled prourokinase release into intercellular space, and the total enzymatic activity of prourokinase does not decrease by more than 12%. The daily release of prourokinase from one square centimeter of the membrane ranges from 1 to 40 μg per day depending on the technique of membrane preparation. The membranes have no acute toxic effect on cells accreting these surfaces de novo. The number of viable cells is at least 96%−97% of the overall cell count. The mitotic index of the cells growing on the surface of the polymeric films comprised around 1.5%. Histological examination did not reveal any disorders in tissues of the animals after the implantation of polymer membranes based on polylactide, both alone and as components of stent cover. Implantation of stents covered with prourokinase-containing polymers led to the formation of a mature connective tissue capsule that is thicker than in the case of uncovered stents. Thus, various polylactide-based biodegradable polymeric membranes possessing the required mechanical properties and capable of prolonged and directed release of prourokinase macromolecules are developed and investigated in the study.
Highlights
The creation of the controlled release and targeting of pharmaceutical agents based on biodegradable polymers is one of the most promising and rapidly growing fields in chemical technology [1]
TheIn incorporation incorporation of into aa polylactide film its to general, elongation of the polylactide films examined linearly depended on the content of prourokinase
General, the elongationThe of elongation of theinto polylactide films film examined linearly depended on the In content of prourokinase
Summary
The creation of the controlled release and targeting of pharmaceutical agents based on biodegradable polymers is one of the most promising and rapidly growing fields in chemical technology [1]. One of the possible areas of application for systems of controlled release and targeting of pharmaceutical agents is their use in coatings of medical devices, especially implants [2]. The product of polycondensation is subjected to chemical treatment and polymerized, yielding a high molar mass polylactide [8]. Polylactide-based polymer films can be used in the creation of dosage formulations [9], in encapsulation technologies [10], sorption [11], target therapy [12], and so forth. Searching and testing new polylactide-based polymeric products is still in progress
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.