Abstract
This study aimed to investigate the production of polyhydroxyalkanoates (PHAs), a biodegradable polymer from organic wastes by Pseudomonas oleovorans. Volatile fatty acids (VFAs) from acidogenic fermentations of chicken manure (VFAs-CM) and potato peels (VFAs-PP), rich in organic matter majorly acetic (49.9%), butyric (15%) and propionic acids (11.1%) were utilized as substrates for microbial processes. During 72 h of cultivations, samples were withdrawn at intervals and analyzed for cell growth parameters, PHAs accumulation and polymeric properties. The highest biopolymer accumulation (0.39 g PHAs/g DCW) was achieved at 48 h of cultivation from medium containing VFAs-PP as the sole source of carbon. On characterization, the produced biopolymers were shown to be semi-crystalline of carbonyl C=O group. Additionally, thermogravimetric analysis (TGA) showed that the produced biopolymers demonstrated the capability to withstand thermal degradation above prescribed temperatures at which cross-linking isomerization reaction occurs, which is a vital property denoting the thermal stability of biopolymer.
Highlights
IntroductionPolyhydroxyalkanoates (PHAs) is a group of biodegradable polymers synthesized by a variety of microorganisms under unbalanced growth conditions as intracellular storage compounds in discrete granular form in their cytoplasm [3]
Production of biodegradable polymer from synthetic volatile fatty acids as well as volatile fatty acids derived from organic wastes by Pseudomonas oleovorans was studied
In the first stage of this work, synthetic volatile fatty acids (SVFAs) were constituted separately into defined media as the sole sources of carbon for cultivation and growth of Pseudomonas oleovorans ATCC 29347 to evaluate its substrate preferences, and feasibility of PHAs accumulation; glucose was used as a reference carbon source (Figure 1a)
Summary
Polyhydroxyalkanoates (PHAs) is a group of biodegradable polymers synthesized by a variety of microorganisms under unbalanced growth conditions as intracellular storage compounds in discrete granular form in their cytoplasm [3]. They possess many advantages compared to synthetic polymers such as hydrophobicity, inertness, thermoplasticprocessability, relatively high melting point, and optical purity [4,5]. The possibility of their production from renewable sources by various microorganisms as well as their biodegradability has further made them attractive alternative to synthetic polymers [6,7].
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