Abstract
Activated sludge contains a versatile microbiome capable of converting wastes into valuable chemicals like polyhydroxyalkanoates (PHA). This study investigated the influence of repeated feast and famine phases on PHA production as well as the corresponding microbial population dynamics using waste activated sludge (WAS) as inoculum. Hydrolysate derived from rice straw was employed as a substrate for PHA production. The 16sRNA analysis results revealed that Corynebacteriaceae (40%), Bacillaceae (23%), and Pseudomonas (5%) were the primary contributors to PHA synthesis. Notably, Bacillaceae and Pseudomonas thrived in all the feast and famine phases. The achieved PHA concentration was 3.5 ± 0.2 g/L, and its structure and composition were assessed using Fourier Transform Infrared Spectroscopy (FTIR) and Nuclear Magnetic Resonance (NMR). The analysis revealed that the PHA consists of a copolymer of hydroxybutyrate (HB) and hydroxyvalerate (HV), specifically identified as Poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV).
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