Polygoni Multiflori Radix Preparat Delays Skin Aging by Inducing Mitophagy.

Xiang Liu,Hongqiu Yang,Ping Liu,Chunyan Yang,Xiaoshuang Du,Yu Du,Ying Deng,Daniele R De Araujo

doi:10.1155/2021/5847153

Xiang Liu, Hongqiu Yang + Show 6 more

Open Access

https://doi.org/10.1155/2021/5847153

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Abstract

Background As the skin is the largest organ of the human body, it is aging inevitably and produces cosmetic and psychological problems, and even disease. Therefore, the molecular mechanisms related to the prevention of skin aging need to be further explored. Methods Aging models were constructed by D-galactose. Mice were administrated with polygoni multiflori radix preparat (PMRP), PMRP and 3-methyladenine, or PMRP and rapamycin intragastrically. The apparent and viscera index of aged rats was measured. Then, the physicochemical property, antioxidant ability, histological structure, mitochondrial membrane potential, ATP and ROS levels, and mitophagy of aged skins were determined. Finally, the expression of PINK1, Parkin, P62, and LC3II/I; apoptosis-related proteins; and the percentage of apoptotic cells were measured. Results PMRP relieved skin aging with reducing of thymus index, improvement of pathological damage and histological structure, increase of the expression area of fibrous tissue, the ratio of type I to type III collagen, and antioxidant ability of aged skins. Importantly, PMRP also improved mitochondrial dysfunction with an increase in the content of mitochondrial membrane potential and ATP and a decrease of ROS levels. Moreover, mitophagy was enhanced with the treatment of PMRP when observed using electron microscopy, and the expression of PINK1, Parkin, and LC3I/II was increased with PMRP treatment but P62 expression was decreased. Meanwhile, PMRP alleviated apoptosis with a decrease of apoptotic cell and the expression of Cleaved-cas3, Bax, Cyt-c, AIF, and Smac as well as an increase of Bcl-2 expression. Conclusion The results demonstrated that the polygoni multiflori radix preparata may delay skin aging by inducing mitophagy.

Highlights

The skin is the most voluminous organ with the largest area of contact with the external environment in the human body [1]
The results suggested that PRMP can improve weight loss caused by aging in the later stages of the trial
The results revealed that the mitochondrial membrane potential in the model group (Model) group was significantly decreased compared with the control group (Control) group, while that in the polygoni multiflori radix preparat (PMRP), PMRP+3-AM, and PMRP+RAPA groups increased significantly compared with the Model group (Figures 6(a) and 6(b))

Summary

Introduction

The skin is the most voluminous organ with the largest area of contact with the external environment in the human body [1]. It provides physical parcloses to the external environment, prevents loss of excessive water, maintains physiological homeostasis, and has certain cosmetic effects [2]. It is urgent to explore the molecular mechanisms related to the prevention of skin aging. The molecular mechanisms related to the prevention of skin aging need to be further explored. The physicochemical property, antioxidant ability, histological structure, mitochondrial membrane potential, ATP and ROS levels, and mitophagy of aged skins were determined. PMRP relieved skin aging with reducing of thymus index, improvement of pathological damage and histological structure, increase of the expression area of fibrous tissue, the ratio of type I to type III collagen, and antioxidant ability of aged skins. The results demonstrated that the polygoni multiflori radix preparata may delay skin aging by inducing mitophagy

Methods

Results

Conclusion