Abstract
Purpose: To investigate the effect of Polygonatum sibiricum extract (PSE) on streptozotocin-induced diabetic rats.
 Methods: PSE was obtained by steeping the dried Polygonatum sibiricum in water at 60 oC three times, each for 1 h, before first drying in an oven at 100C and then freeze-drying the final extract, thus obtained. Diabetic model rats were prepared by a single intraperitoneal injection of a freshly prepared solution of streptozotocin (50 mg/kg). The rats were randomly divided into 6 groups of ten rats each: negative control, normal control, reference (glibenclamide1 mg/kg) as well as PSE groups, (35, 70 and 140 mg/kg). Blood glucose and plasma insulin levels were measured to determine antihyperglycemic effect. Oxidative stress was evaluated in liver and kidney by their antioxidant markers, viz, lipid peroxidation (LPO), superoxide dismutase (SOD), reduced glutathione (GSH), glutathione peroxidase (GPx) and catalase (CAT). Blood serum levels of creatinine and urea were determined in both diabetic control and treated rats.
 Results: Compared with diabetic rats, oral administration of PSE at a concentration of 120 mg/kg daily for 30 days showed a significant decrease in fasting blood glucose (118.34 ± 3.29 mg/dL) (p < 0.05) and increased insulin level (12.86± 0.62 uU/mL, p < 0.05). Furthermore, it significantly reduced biochemical parameters (serum creatinine, 0.83 ±0.21 mg/dL, p < 0.05) and serum urea (43.26±1.42 mg/dL, p < 0.05).
 Conclusion: The results suggest that PSE may effectively normalize impaired antioxidant status in streptozotocin-induced diabetes in a dose-dependent manner. Thus, PSE has a protective effect against lipid peroxidation by scavenging free radicals, restoration of insulin function, and reduction of the incidence of complications.
Highlights
Diabetes mellitus is a metabolic disease that manifests due to insulin insufficiency and/or insulin resistance and has become a serious health problem worldwide
Group I served as control and received distilled water
Group II served as diabetic control and received distilled water
Summary
Diabetes mellitus is a metabolic disease that manifests due to insulin insufficiency and/or insulin resistance and has become a serious health problem worldwide. The increased blood glucose levels in diabetes produce superoxide anions, which generate hydroxyl radicals via Haber–Weiss reaction, resulting in peroxidation of membrane lipids and protein glycation. This leads to oxidative damage of cell membranes. The rat experiment was approved by the Animal Care and Use Committee of Shandong University of Chinese medicine Taxonomic identification of the plant was performed by Professor Ling Hu of Shandong University of Chinese medicine in China. Rats were fasted overnight and the blood was withdrawn by retro orbital puncture under light ether anesthesia on the 1st, 15nd and 30th day post induction to determine blood glucose and plasma insulin level. All biochemical tests were carried out in our lab by using commercial kits obtained from Erba diagnostic Mannheim Gmbh, Germany
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