Abstract
A new class of fluorocarbon affinity resins for the separation and purification of immunoglobulins, antibodies, and other biomolecules has been introduced by Du Pont under the trade name Pedlex®. The discovery that proteins fluoroalkylated by n-C 8F 17CH 2CH 2CH 2NCO bind strongly to fluorocarbon surfaces underlies this technology. The resins work well with proteins, but enzymes invariably lose activity when adsorbed. Moreover, their use is restricted to aqueous media since organic solvents wash the fluoroalkylated proteins off the support. Tetra-, penta-, and hexa(polyfluoroalkyl) sugar reagents ( 1, 2, 3 R CO(CH 2) m(CF 2) nCF 3) that overcome these limitations have been synthesized. Their preparation, model binding studies, and use in immobilizing chymotripsin and protein A on Teflon® will be discussed. ▪
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