Abstract
In this work, the positively-charged polymer polyethyleneimine was used to functionalize carbon nanotubes and activated carbon to load antimycotic enzyme lyticase. Interestingly, polyethyleneimine played a dual role functionalizing carbon materials to synergistically enhance antimycotic activity of loaded lyticase as well as exhibiting its own apparent antimycotic activity, where the enhanced enzymatic activity of loaded lyticase on functionalized carbon nanotubes was more than 2.8 times as high as the activity of free enzyme in solution. The actual activity of loaded lyticase on functionalized carbon nanotubes was applied with Penicillium janthinellum, exhibiting much faster digesting lysis of the bacteria in comparison with free lyticase. The synergistic and potent antimycotic activities from combined action of antimycotic lyticase and polyethyleneimine on carbon nanotubes provides a new antimycotic protection for medicine, food industry, and other biochemical processes.
Highlights
Carbon Nanotubes Enabling PotentPolyethyleneimine (PEI) is a linear or branched or dendrimeric synthetic polymer with repeating unit composed of the amine groups and two carbon aliphatic CH2 CH2 spacer
Polyethyleneimine (PEI) is a linear or branched or dendrimeric synthetic polymer with repeating unit composed of the amine groups and two carbon aliphatic CH2 CH2 spacer
The carbon materials were incubated in the the working buffer, we found there was no enzyme desorption/leaking, enzyme stock solution, thereby avoiding harsh immobilization conditions thatbut destroy indicating that the strong adsorption is not attributed to electrostatic interaction, enzymatic activity. interaction
Summary
Carbon Nanotubes Enabling PotentPolyethyleneimine (PEI) is a linear or branched or dendrimeric synthetic polymer with repeating unit composed of the amine groups and two carbon aliphatic CH2 CH2 spacer. We measured the protein-loading density of the enzyme on CNT and determined the specific (antimycotic) activity of the loaded LYT and the apparent antimycotic activity of the carbon materials in the absence of LYT in comparison with that of the free lysozyme in solution.
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