Polyethylene Oxide Assisted Fish Collagen-Poly-ε-Caprolactone Nanofiber Membranes by Electrospinning.

Xiaoli He,Wenjun Li,Song Qin,Zhihong Tang,Lei Wang,Kangning Lv

doi:10.3390/nano12060900

Abstract

Fish collagen has higher biocompatibility and lower immunogenicity than terrestrial collagen, and is currently one of the important raw materials for preparing biological materials. In this study, PEO was used as a spinning aid to prepare fish skin collagen-PCL nanofiber membranes by electrospinning, and the process was optimized to get smooth nanofibers. The morphological and mechanical properties of collagen-PCL nanofiber membranes were assessed by scanning electron microscopy (SEM). The changes in chemical composition due to the incorporation of collagen into PCL and PEO were determined by Fourier Transform infrared spectroscopy (FTIR). The biocompatibility of the collagen-PCL nanofiber membranes was evaluated in vitro in cultures of mouse fibroblasts and in vivo by subcutaneous implantation studies in rats. It was found that the diameter of the spun fibers became fine and smooth when the ratio of the collagen/PCL increased. The finally obtained nanofiber had good mechanical strength, porosity, and hydrophilicity, and could promote cell adhesion and proliferation. The FC-PCL nanofiber membrane prepared by this route opens a new way to prepare fish collagen biomaterials with electrospinning.

Highlights

Collagen is the most abundant protein in the human body and has become one of the most widely used materials in tissue engineering due to its superior biological properties [1].Compared with land-based collagen, collagen derived from aquatic animals has the advantages of a wide range of sources, low production cost, low immunogenicity, and is free from the influence of mad cow disease, religion, and other factors [2], so it has been applied more and more widely in the field of materials
Fish collagen was blended with PCL by electrospinning, and the nanofiber membrane material based on fish collagen was successfully prepared
The characterization and biological test of the material showed that the material had good mechanical properties and biocompatibility, can promote cell adhesion and proliferation, and can be used as a potential medical material for tissue defect and wound repair

Summary

Introduction

Collagen is the most abundant protein in the human body and has become one of the most widely used materials in tissue engineering due to its superior biological properties [1].Compared with land-based collagen, collagen derived from aquatic animals has the advantages of a wide range of sources, low production cost, low immunogenicity, and is free from the influence of mad cow disease, religion, and other factors [2], so it has been applied more and more widely in the field of materials. Collagen is the most abundant protein in the human body and has become one of the most widely used materials in tissue engineering due to its superior biological properties [1]. Tilapia (Oreochromis mossambicus) is a common freshwater fish in China. The processing of Tilapia can produce a lot of fish skin, which will pollute the environment if it is not handled properly. The extraction of collagen from Tilapia skin (FC) and further development and utilization can increase the added value of products, and avoid resource waste and reduce environmental pollution [2]. It has been reported that the Tilapia skin collagen film-forming property is significantly better than that of freshwater fish such as carp (Hypophthalmichthys molitrix) and grass carp (Ctenopharyngodon idella) [3]

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