Polyethylene nanoparticles at environmentally relevant concentrations enhances neurotoxicity and accumulation of 6-PPD quinone in Caenorhabditis elegans

Abstract

The exposure risk of 6-PPD quinone (6-PPDQ) has aroused increasing concern. In the natural environment, 6-PPDQ could interact with other pollutants, posing more severe environmental problems and toxicity to organisms. We here examined the effect of polyethylene nanoplastic (PE-NP) on 6-PPDQ neurotoxicity and the underling mechanisms in Caenorhabditis elegans. In nematodes, PE-NP (1 and 10 μg/L) decreased locomotion behavior, but did not affect development of D-type neurons. Exposure to PE-NP (1 and 10 μg/L) strengthened neurotoxicity of 6-PPDQ (10 μg/L) on the aspect of locomotion and neurodegeneration induction of D-type motor neurons. Exposure to PE-NPs (10 μg/L) caused increase in expressions of mec-4, asp-3, and asp-4 governing neurodegeneration in 10 μg/L 6-PPDQ exposed nematodes. Moreover, exposure to PE-NP (10 μg/L) increased expression of some neuronal genes (daf-7, dbl-1, jnk-1, and mpk-1) in 6-PPDQ exposed nematodes, and RNAi of these genes resulted in susceptibility to neurotoxicity of PE-NP and 6-PPDQ. 6-PPDQ could be adsorbed by PE-NPs, and resuspension of PE-NP and 6-PPDQ after adsorption equilibrium exhibited similar neurotoxicity to co-exposure of PE-NP and 6-PPDQ. In addition, exposure to PE-NP (1 and 10 μg/L) increased 6-PPDQ accumulation in body of nematodes and increased defecation cycle length in 6-PPDQ exposed nematodes. Therefore, 6-PPDQ could be adsorbed on nanoplastics (such as PE-NPs) and enhance both neurotoxicity and accumulation of 6-PPDQ in organisms.