Abstract
SUMMARY In attempts to infect cucumber protoplasts with cucumber mosaic virus (CMV) RNA, the use of polyethylene glycol as an uptake inducer proved successful. This method involves resuspending protoplasts in high concentrations of polyethylene glycol in the presence of RNA, followed by rapid dilution. Levels of infection obtained with CMV-RNA were more reproducible than those using virus nucleoprotein. This method was also suitable for the inoculation of protoplasts with tobacco ringspot virus RNA.
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