Abstract
We report a design and fabricate multifunctional localized platform for cancer therapy. Multiple stimuli-responsive polydopamine (PDA) was used for surface modification of electrospun doxorubicin hydrochloride (DOX) loaded polycaprolactone (PCL) fibers to make a designated platform. Photothermal properties such as photothermal performance and stability of the resulting composite mats were studied under the irradiation of the near-infrared (NIR) laser of 808 nm. With the incorporation of PDA into the fiber, a remarkable increase of local temperature was recorded under NIR illumination in a concentration-dependent manner with excellent stability. Drug released assay results revealed PDA coated PCL-DOX mats showed pH and NIR dual responsive behavior thereby exhibiting improved drug release in an acidic medium compared to physiological pH condition (pH 7.4) which is further increased by NIR exposure. The cancer activity in vitro of the mats was evaluated using cell counting (CCK) and live and dead cell assays. The combined effect of NIR mediated hyperthermia and chemo release resulting improved cells death has been reported. In summary, this study presents a major step forward towards a therapeutic model to cancer treatment utilizing pH and NIR dual responsive property from PDA alone in a fibrous mat.
Highlights
Cancer tissue offers different microenvironmental conditions compared to adjacent normal cells or tissues in terms of pH, oxygen level, and temperature[11]
The present study aims to develop smart multiple stimuli-responsive nanofibers that are capable of both pH/NIR dependent drug release in a cancer environment and heat generation under the application of NIR triggered phototherapy for the combined treatments of hyperthermia and chemotherapy
We have reported the successful fabrication of localized anticancer drug delivery platform with bimodal functionalities
Summary
Fabrication and Characterization of PDA Coated Mats. In this work, we chose the PCL as a model polymer to make an electrospun fiber, a drug carrier, due to its abundant uses in biomedical applications including drug delivery. In the same period (2 weeks) PDP2 in pH 7.4 showed the lowest value which is 16 and 7% lower than its counterpart mat that had conducted at pH 5.5 and 6.8, respectively (Fig. 6A) These results indicate clearly the drug release from both fibers coated and uncoated is dependent to the pH of the PBS solution. PDP0 exhibited a significant reduction of cell viability compared to not irradiated PDP2 mats or PDP2 mats with irradiation by 1 W/cm[2] (Fig. 7B) This result is accompanied by the burst release of DOX from PDP0 fiber into the cell medium, which directly induced cell deaths while PDA coated mats not subjected to NIR resulted in no apparent toxicity which is attributed to the coating of the biocompatible, non-toxic PDA over the drug-loaded mats. In-vitro cells results indicated that PDA decoration into the drug-loaded fibers could be an effective localized platform that can inhibit tumor cell growth and eventual killing by delivering both a hyperthermia and heat mediated drug delivery under NIR exposure
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