Abstract
Silver nanoparticles (AgNPs) are extensively applied for their broad-spectrum and excellent antibacterial ability in recent years. Polydopamine (PDA) has great advantages for synthesizing large amounts of AgNPs, as it has multiple sites for silver ion binding and phenolic hydroxyl structure to reduce silver ions to AgNPs. Here, we mixed sericin and agar solution and dried at 65 °C to prepare a sericin (SS)/Agar composite film, and then coated polydopamine (PDA) on the surface of SS/Agar film by soaking SS/Agar film into polydopamine solution, subsequently synthesizing high-density AgNPs with the assistance of PDA to yield antibacterial AgNPs-PDA- SS/Agar film. Scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FT-IR) and X-ray diffraction (XRD) spectra indicated the successful synthesis of high-density AgNPs on the surface of PDA-SS/Agar film. PDA coating and AgNPs modification did not affect the structure of sericin and agar. Furthermore, water contact angle, water absorption and mechanical property analysis showed that AgNPs-PDA-SS/Agar film had excellent hydrophilicity and proper mechanical properties. Inhibition zone and growth curve assays suggested the prepared film had excellent and long-lasting antibacterial ability. In addition, it had excellent cytocompatibility on the fibroblast NIH/3T3 cells. The film shows great potential as a novel kind of wound dressing.
Highlights
The antibacterial ability of a material surface is crucial to inhibiting the growth of bacteria on and around the material, which has great potential in food packaging and biomedical application [1]
We used PDA to direct the synthesis of the antibacterial AgNPs on the surface of PDA-coated Silk sericin (SS)/Agar film
SS/Agar film was immersed into dopamine solution to produce PDA-coated SS/Agar film
Summary
The antibacterial ability of a material surface is crucial to inhibiting the growth of bacteria on and around the material, which has great potential in food packaging and biomedical application [1]. The moisturizing property, the ability of promoting epithelial cell growth and oxidation resistance mean that sericin possesses great potential in biomedical applications. Chlapanidas et al studied the biological properties of silk fibroin from 20 strains, and picked the most promising strains in which sericin was developed for cosmetic and dermatological applications [9]. They showed that sericin microspheres loaded with tumor necrosis factor-α (TNF-α) blockers contribute to the down-regulation of cytokines [10]. It is important to find a substance which could improve the density of AgNPs, and increase the hydrophilicity of the material surface for biomaterial application. The issue of whether PDA can produce biologically active dopamine in vivo has not been well addressed in the literature on PDA-modified materials
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