Abstract
Polydatin, an active ingredient from the roots of Polygonum cuspidatum, is considered to have protective effects on the cardiovascular system and liver. In this study, we demonstrated that polydatin has antitumor activity against human cervical cancer. Polydatin efficiently inhibited cervical cancer cell proliferation by regulating cell cycle-related proteins including p21, p27, CDK2, CDK4, Cyclin D1, and Cyclin E1. Furthermore, polydatin suppressed cell invasion and migration by regulating epithelial–mesenchymal transition (EMT) markers, including E-cadherin, N-cadherin, Snail and Slug. The c-Myc, as a proto-oncogene, is considered to be closely associated with the proliferation and metastasis of tumor cells. After polydatin treatment, the protein expression of c-Myc showed a significant decrease. Based on these data, we overexpressed c-Myc in cervical cancer cells and observed that the overexpression of c-Myc rescued the inhibitory effect of polydatin on cell proliferation and metastasis. These results indicated that polydatin can inhibit cell proliferation and metastasis through suppressing the c-Myc expression in human cervical cancer.
Highlights
MATERIALS AND METHODSAmong females, cervical cancer is the fourth most common malignancy in terms of incidence and mortality (Bray et al, 2018)
To evaluate the inhibitory effect of PD on the proliferation of human cervical cancer cells, CaSki and C33A cells were treated with different concentrations of PD for 72 h, and the results showed that the cell proliferation was inhibited in a dose-dependent manner (Figure 1A)
These results described above indicated that PD markedly inhibited cervical cancer cell growth and proliferation
Summary
Cervical cancer is the fourth most common malignancy in terms of incidence and mortality (Bray et al, 2018). In human hepatocellular carcinoma cells, resveratrol suppressed invasion of cells through inhibition of tumor necrosis factor-α-mediated matrix metalloproteinase 9 (MMP9) expression (Yu et al, 2008) and caused cell cycle arrest via the activation of the p53-dependent pathway in colorectal cancer (Liu et al, 2019) These lines of evidence significantly suggested that stilbenes are promising candidates for tumor therapy. Our studies indicated that PD inhibited the proliferation, cell cycle progression, and migration/invasion of cervical cancer cells by mediating the c-Myc pathway. These findings suggested that PD may act as a potential candidate drug for the treatment of cervical cancer. After being treated with DMSO or PD at indicated concentrations, human cervical cancer cells were harvested, and the Western blot assay was performed as previously described (Zhang et al, 2018). Differences between means were determined via unpaired Student’s t-tests, and P < 0.05 was considered statistically significant
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
