Polycyclic aromatic hydrocarbons degradation by Agrocybe sp. CU-43 and its fluorene transformation

Abstract

Agrocybe sp. CU-43, a white-rot fungus isolated from Thailand, showed a high potential for degrading both low- and high-molecular weight polycyclic aromatic hydrocarbons. At 100 ppm fluorene was degraded by 99% within six days while at the same concentration 99 and 92% degradation of phenanthrene and anthracene, respectively, occurred in 21 days, and fluoranthene and pyrene were reduced by 80 and 75%, respectively, in 30 days. In a soil model, Agrocybe sp. CU-43 completely degraded 250 ppm fluorene at room temperature within four weeks. Laccase and manganese peroxidase activities, but not lignin peroxidase activity, were detected during the biodegradation of fluorene. Two of the metabolites from fluorene degradation by the fungus were identified via reversed-phase HPLC as 9-fluorenol and 9-fluorenone, the less toxic intermediates of fluorene. However, 9-fluorenol is not an end product for the degradation. These results suggest that fluorene degradation by Agrocybe sp. CU-43 may take place via the same pathway(s) employed by other ligninolytic and non-ligninolytic fungi. This is the first report of fluorene biodegradation by a fungus belonging to the genus Agrocybe.