Abstract
Spleens of C57B1 6 mice inoculated im with the Moloney murine sarcoma-leukemia virus (MSV) complex were assayed for the formation of plaque-forming cells (PFC) against SRBC, TNP-SRBC, and FITC-SRBC. Within a few days after virus inoculation, the number of PFC against each one of these antigens increased markedly reaching peak levels on Day 10. Polyclonal antibody formation of similar magnitude was also observed after MSV inoculation of either C57B1 6 athymic nu nu mice or C3H HeJ mice. No PFC response was detected in mice inoculated with virus preparations which had been exposed to ultraviolet light. These results thus indicate that polyclonal B-cell activation occurs in spleens of MSV inoculated mice, and that this response (a) does not require the presence of T cells, (b) depends on virus replication in vivo, and (c) is not related to the presence of contaminating bacterial lipopolysaccharide in the virus preparation. Autoantibodies against bromelain-treated syngeneic red blood cells and against thymocytes were also detected in MSV-inoculated mice. The kinetics of their formation was found to parallel the development of the polyclonal antibody response.
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