Polychlorobiphenyls and other organochlorine compounds in human follicular fluid

Abstract

Based on observations in animals, there is an increasing evidence that a number of persistent organochlorine pollutants can alter the endocrine homeostasis, this resulting in toxic effects in particular in the developing organism. However, the role of these chemicals in determining endocrine-related diseases in humans, and possibly a decrease of fertility, is still controversial. Exposure data concerning the human reproductive system are essential for risk assessment. Based on this, the occurrence in follicular fluid of polychlorobiphenyls (PCBs), 2,3,7,8-chlorosubstituted polychlorinated dibenzo- p-dioxins (PCDDs) and dibenzofurans (PCDFs), 1,1,1-trichloro-2,2- bis(4-chlorophenyl)-ethane ( p, p ′-DDT) and its metabolites, and hexachlorobenzene (HCB) was investigated. With respect to PCBs, the sum of the three most abundant congeners (PCBs 138, 153 and 180) was 1230 ng/g, lipid basis (0.37 ng/g, wet weight). Congener distribution profile overlapped what is usually observed in other human tissues, as blood and milk. PCDDs, PCDFs, p, p ′-DDT and 1,1-dichloro-2,2- bis(4-chlorophenyl)-ethane ( p, p ′-DDD) were below their determination limits. 1,1-dichloro-2,2- bis(4-chlorophenyl)-ethene ( p, p ′-DDE) and HCB were detected in concentrations respectively in the order of 700 and 70 ng/g, lipid basis (≈0.2 and ≈0.02 ng/g, wet weight).