Abstract
Detection of aflatoxin B1 (AFB1) in environmental samples and body fluids is necessary for taking effective precautions and treatment of contamination. Its invisible presence is also associated with huge economic losses to nations. Plastic optical fiber (POF) offers a cost-effective yet flexible waveguide for visible light to develop optical biosensors. We have developed an ultrasensitive POF based AFB1 sensor that operates from pico to micromolar concentration with reasonable accuracy (R2 = 0.99). AntiAFB1 antibody has been used as bioreceptor for the sensor. The optical property of polyaniline (PAni) has been explored on polymethylmethacrylate (PMMA) surfaces of POF for the fabrication of a simple, inexpensive, yet highly sensitive AFB1 biosensor. The evanescent wave absorbance-based sensor was able to detect analytes below the maximum permissible residual limit (MRL) set by any country for spiked peanuts, corn, wheat, and beer. The sensor was further optimized to detect AFB1 in spiked human serum and urine within the pathological relevance. The recovery results fell between 81 % and 117 %, demonstrating reasonable accuracy. The matrix factor of different real matrices lies within around 0.85–1.14, i.e., within the acceptable range.
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