Abstract
The polyamine (PA) concentration was greatest in the nodules at stage 2 (flowering) in Vigna mungo. The amount of putrescine (Put) was the highest and changed significantly with senescence, whereas the spermine (Spm) level was the least. During senescence, both arginine decarboxylase (ADC, EC 4.1.1.19) and ornithine decarboxylase (ODC, EC 4.1.1.17) were positively correlated with the endogenous PA titre, particularly in the late stages of development, while the catabolic enzyme, diamine oxidase (DAO, EC 1.4.3.6) increased with senescence, being highest (five-fold) in nodules at stage 5 (i.e. mature pod stage). In vivo labelling studies using l-[U- 14C]arginine with difluoromethylarginine (DFMA) and difluoromethylornithine (DFMO) indicated that both Put biosynthetic enzymes are involved during the process of senescence. Moreover, Spm promoted nitrogenase activity in vivo.
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