Polyamine metabolism in enterocytes isolated from newborn pigs

Abstract

In the pig, the growth of intestinal mucosa is very intense after birth. Since the polyamines are key elements affecting cell proliferation and differentiation, the present work was undertaken in order to know whether this hypertrophy is associated with an adaptation of polyamine metabolism. Villus enterocytes isolated from pig immediately after birth or 2 days later were found to contain similar amounts of putrescine, spermidine and spermine, i.e., 0.23; 0.41 and 1.24 nmol 10 6 cells , respectively. At birth, despite a relatively high ODC activity, putrescine synthesis from 1 mM l-arginine or 2 mM l-glutamine was very low in isolated enterocytes ( 6.4 ± 3.8 pmol 10 6 cells per 30 min), while spermidine and spermine production were not detectable. This could be explained by a very low l-ornithine generation from both amino acids and to an inhibitory effect of polyamines on ODC activity. Two days later, polyamine synthesis from l-arginine remained undetectable despite a higher l-ornithine generation. This was concomitant with a dramatic fall in ODC activity. At both stages, enterocytes were able to take up polyamines from the extracellular medium in a temperature-dependent manner. It is concluded that de-novo synthesis of polyamines from l-arginine or l-glutamine does not play a significant role in the control of polyamine content of pig enterocytes during the postnatal period. In contrast, polyamine uptake by enterocytes would contribute to maintain a steady-state polyamine content during this period.