Polyadenylated tail length variation pattern in ultra-rapid vitrified bovine oocytes.

D J Dutta,Himangshu Raj,Hiramoni Dev

doi:10.14202/vetworld.2016.1070-1074

D J Dutta, Himangshu Raj + Show 1 more

Open Access

https://doi.org/10.14202/vetworld.2016.1070-1074

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Journal: Veterinary world	Publication Date: Oct 1, 2016
Citations: 2	License type: cc-by

Affiliation: Assam Agricultural University

Abstract

Aim:Thecurrent study aims at investigating the polyadenylated (poly[A]) tail length of morphologically high and low competent oocytes at different developmental stages. Furthermore, effect of ultra-rapid vitrification on the poly(A) tail length was studied.Materials and Methods:Fresh bovine cumulus oocyte complexes from abattoir originated ovaries were graded based on morphological characters and matured in vitro. Cryopreservation was done by ultra-rapid vitrification method. mRNA was isolated from different categories of oocyte and subjected to ligation-mediated poly(A) test followed by polymerase chain reaction for determining the poly(A) tail length of β actin, gap junction protein alpha 1 (GJA1), poly(A) polymerase alpha (PAPOLA), and heat shock 70 kDa protein (HSP70) transcripts.Results:GJA1, PAPOLA, and HSP70 showed significantly higher poly(A) in immature oocytes of higher competence irrespective of vitrification effects as compared to mature oocytes of higher competence.Conclusion:mRNA poly(A) tail size increases in developmentally high competent immature bovine oocytes. There was limited effect of ultra-rapid vitrification of bovine oocytes on poly(A).

Highlights

The polyadenylated tail on most eukaryotic mRNAs plays a number of important roles in mRNA metabolism including regulating translation, mRNA stability, and transport from the nucleus
Cryopreservation was done by ultra-rapid vitrification method. mRNA was isolated from different categories of oocyte and subjected to ligation-mediated poly(A) test followed by polymerase chain reaction for determining the poly(A) tail length of β actin, gap junction protein alpha 1 (GJA1), poly(A) polymerase alpha (PAPOLA), and heat shock 70 kDa protein (HSP70) transcripts
Conclusion: mRNA poly(A) tail size increases in developmentally high competent immature bovine oocytes

Summary

Introduction

The polyadenylated tail (poly[A] tail) on most eukaryotic mRNAs plays a number of important roles in mRNA metabolism including regulating translation, mRNA stability, and transport from the nucleus. Poly(A) plays a key regulatory step in gene expression and is known to be important for the developmental competence of oocyte and early embryonic development [1,2]. Poly(A) tail length regulation has been shown to play a key role in some biological processes, such as oocyte maturation, mitotic cell cycle progression [4,5,6]. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/ publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated

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