Abstract
Enzymes, antibodies, lectins, and small ligands were coupled to glutaraldehyde-activated polyacrylhydrazido-agarose. High yields of binding and activity were obtained. The bound enzymes exhibited increased stability to heat and 6 M urea when compared with the respective soluble enzyme as well as enzymes coupled to cyanogen bromide-activated agarose. Antibodies and lectins were also found to maintain specificity for the appropriate antigens and glycoprotein receptors, and were used effectively for affinity chromatography purifications. Polyacrylhydrazido-agarose provides a new carrier that combines advantages of both agarose and acrylamide in one resin.
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