Abstract
AbstractPolyacrylamide Gel Electrophoresis is based on the principle of migration of charged particles under the influence of electric field to separate out proteins and nucleic acids. This chapter outlines this technique with respect to the separation of milk proteins along with the most frequently used protocols of gel media, buffer system, sample preparation, gel staining and visualization techniques. There are three commonly used PAGE techniques, namely, native PAGE (based on net charge, size, and shape of proteins), SDS PAGE (based on size of proteins), and urea PAGE (based on net negative charge and size of proteins) which can be utilized for various applications during analysis of milk proteins. Moreover, selection of correct visualization and detection techniques after separation of proteins over gels is as important as the protocol itself during electrophoresis.KeywordsNative PAGESDS PAGEUrea PAGECaseinWhey proteinCBB staining
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