Abstract
A critical account of factors affecting standardization and performance of polyacrylamide gel electrophoresis is given (sample preparation, apparatus operation, gel formation, gel testing, quantitative evaluation); some recent advances of the method are described (two-dimensional techniques; gradient gel electrophoresis; enzyme assays; tissue extraction procedures) and some problems related to optimal fractionation are discussed (electrochemistry of multiphasic buffer systems as related to ionic strength and Joule heating, pulsed constant electric power and Joule heating; zone curvature; catalyst artifacts and apparatus-inherent problems).
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