Abstract
Poly-3-hydroxyalkanoates (PHAs) are biopolymers accumulated as intracellular energy reserves by bacteria under nutrient limiting growth conditions and suitable for plastic production. We report findings from the use of a bacterium isolated from a cassava dumpsite for PHAs production. Isolate C113 was screened for PHAs production using the viable colony and Sudan Black B staining methods and further identified by 16SrRNA sequencing. Its PHA synthase gene, PhaR , was also partially amplified and sequenced. PHAs production was achieved over 96-hour incubation, extracted by NaClO/chloroform method and analysed by FT-IR. Isolate C113 was positive for PHA production in all carbon sources and was identified as a strain of Bacillus cereus . It showed highest biomass accumulation in glucose and starch. It achieved PHAs production of 4.85 % dry cell weight (DCW) in glucose, 33.03 %DCW in glycerol and 6.05 %DCW in sugarcane molasses at 24 hours while 10.58 %DCW was produced in starch at 96 hours. FT-IR spectra showed peaks corresponding to P3HB and P3HB3HV and reveal conformational changes of mcl-PHA and scl-PHA in crystalline and amorphous phases. The 16SrRNA (KY855372.1) and PhaR (MF947451.1) sequences have been accessioned in NCBIGenBank. The results show that Bacillus cereus C113 demonstrated capacity to utilize a variety of carbon sources for PHAs production.
Highlights
Poly-3-hydroxyalkanoates (PHAs) are biodegradable elastomers that show valuable material properties (Spiekermann et al, 1999; Babruwad et al, 2015)
The class IV synthases chain length (MCL) containing 6 – 14 carbon are encoded by two genes, PhaC and PhaR, and atoms and long chain length (LCL) containing ≥ both are important for PHA polymerization
Following 16SrRNA sequencing, the organism was identified as Bacillus cereus strain C113 (NCBI Accession number: KY855372.1) sharing closest homology with B. cereus JCM 2152 at 99%
Summary
Poly-3-hydroxyalkanoates (PHAs) are biodegradable elastomers that show valuable material properties (Spiekermann et al, 1999; Babruwad et al, 2015). B. cereus PS-10 utilized 3% w/v molasses and 2% v/v glycerol as carbon substrates to produce 9.5 g/l and 8.9 g/l PHB as reported by Sharma and Bajaj (2015). Our study reports the use of a new strain of Bacillus cereus isolated from cassava dumpsite with potential for sustainable PHAs production.
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